Journal: Materials today. Bio

Article Title: Precise delivery of doxorubicin and imiquimod through pH-responsive tumor microenvironment-active targeting micelles for chemo- and immunotherapy.

doi: 10.1016/j.mtbio.2022.100482

Figure Lengend Snippet: Fig. 10. Immunostaining of tumor tissues in tumor-bearing mice after treatments. (A) Immunohistochemistry images of tumor sections stained with CD3, CD8, and TNF-α antibodies. The scale bar is 100 μm. (B) Immunofluorescence images of tumor tissues after treatments for 12 days. The scale bar is 50 μm. Blue fluorescence represents the cell nucleus stained with DAPI. Green fluorescence represents the iNOS stained with the iNOS antibody conjugated with FITC.

Article Snippet: After 30 min, the tissue slice was stained with diluted iNOS antibody (Miltenyi Biotec, catalog: 130-116-357) at 4 C overnight.

Techniques: Immunostaining, Immunohistochemistry, Staining

Journal: Clinical and Experimental Pharmacology & Physiology

Article Title: Mechanisms of IL‐17A Neutralisation in Alleviating Renal Fibrosis and Inflammation in Spontaneously Hypertensive Rats

doi: 10.1111/1440-1681.70116

Figure Lengend Snippet: Effects of IL‐17A neutralisation on macrophage polarisation in SHR renal tissues. (A) Representative IHC staining of M1 macrophage markers (iNOS and CD86) with quantitative analysis of their positive areas. (B) Representative IHC staining of M2 macrophage markers (Arg‐1 and CD163) with quantitative analysis of their positive areas. (C) Representative immunoblots and relative expression levels of iNOS, CD86, Arg‐1, and CD163 proteins. (D) Proportion of CD86 + and CD163 + cells among CD68 + macrophages. (E) mRNA expression levels of iNOS, CD86, Arg‐1, and CD163. Data are presented as mean ± SD ( n = 6).

Article Snippet: Sections were then incubated overnight at 4°C with primary antibodies against: E‐cadherin (Boster, China), Collagen III (Boster, China), inducible nitric oxide synthase (iNOS) (Boster, China), CD86 (Boster, China), arginase‐1 (Arg‐1) (Boster, China), CD163 (Boster, China), α‐smooth muscle actin (α‐SMA) (Cell Signalling Technology, USA).

Techniques: Immunohistochemistry, Western Blot, Expressing

Journal: PLoS ONE

Article Title: Heading towards a dead end: The role of DND1 in germ line differentiation of human iPSCs

doi: 10.1371/journal.pone.0258427

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: NANOG , REA314 , PE , Recombinant human IgG1 , anti-human , 130-117-526 , Miltenyi Biotec.

Techniques: Cytometry, Conjugation Assay, Recombinant, Control

Journal: International journal of molecular medicine

Article Title: Fasudil, a Rho-associated protein kinase inhibitor, attenuates retinal ischemia and reperfusion injury in rats.

doi: 10.3892/ijmm.2011.659

Figure Lengend Snippet: Figure 5. Immunohistochemical staining of iNOS in rat retinas at 1 day after I/R. (A) sham group; (B) I/R group; (C) control group; (D) fasudil group. The rats in I/R group and control group exhibited a remarkable increase in iNOS reactivity (n=5).

Article Snippet: A rabbit polyclonal antibody against rat iNOS (1:100, Boster Bio-Engineering co. Ltd., Wuhan, china) was used as a primary antibody.

Techniques: Immunohistochemical staining, Staining, Control

Journal: International journal of molecular medicine

Article Title: Fasudil, a Rho-associated protein kinase inhibitor, attenuates retinal ischemia and reperfusion injury in rats.

doi: 10.3892/ijmm.2011.659

Figure Lengend Snippet: Figure 6. iNOS mRNA and protein levels in rat retinas at 1 day after I/R were measured by quantitative real-time RT-PCR (A) and Western blot analysis (B), respectively. (A) Compared to the sham group, there was a marked increase in iNOS mRNA in the I/R group and the control group. Fasudil pre-treatment significantly attenuated the increase of iNOS mRNA in retinas of rats with I/R. (B) iNOS protein levels in the above-mentioned groups were consistent with the mRNA expression by Western blotting. Representative blots are shown, and the protein size is expressed in kDa. (n=5, *P<0.05 vs. the sham group, #P<0.01 vs. the control group).

Article Snippet: A rabbit polyclonal antibody against rat iNOS (1:100, Boster Bio-Engineering co. Ltd., Wuhan, china) was used as a primary antibody.

Techniques: Quantitative RT-PCR, Western Blot, Control, Expressing